In vitro propagation and microtuber induction in Dioscorea belophylla (Prain) Voigt ex Haine

Authors

  • Sreeja Thankappan Central Tuber Crops Research Institute (ICAR-CTCRI), Sreekariyam, Thiruvananthapuram - 695017, Kerala, India
  • Abraham K Central Tuber Crops Research Institute (ICAR-CTCRI), Sreekariyam, Thiruvananthapuram - 695017, Kerala, India

Keywords:

Dioscorea belophylla, in vitro propagation, microtuber induction, BAP, Kin, NAA

Abstract

A protocol was developed for the in vitro propagation and microtuber induction in Dioscorea belophylla  using nodal explants at Central Tuber Crops Research Institute, Thiruvananthapuram, India. MS  medium with benzyl adenine (BA) at 3 μM and Kinetin (Kin) at 4 and 5 μM were found to be the best  concentrations for developing shoots with good number of nodes and leaves. The addition of 1-naphthaleneacetic acid (NAA) along with cytokinin resulted in healthier leaf production. Among the  different concentrations of sucrose tested, MS medium with 4% sucrose was the best for shooting,  having the highest shoot length of 11.8 cm and mean number of 17.4 nodes and 11.4 leaves/shootwithin 15.4 days of inoculation. Microtuber induction was recorded in the presence of BA (3-5 μM),  Kin (3-4 μM) and BA 3 μM along with NAA (1 μM). Addition of 3-8% sucrose in the medium was found  to be the best concentration for inducing 1-3 microtubers/culture.

References

Acedo, V.Z. 2003. Propagation of purple yam (Dioscorea alata) through tissue culture. J. Root Crops, 29 (2): 7-16.

Alizadeh, S., Mantell, S.H. and Viana, A.M. 1998. In vitro shoot culture and microtuber induction in the steroid yam Dioscorea

composita Hemsl. Plant Cell Tissue Organ Cult., 53: 107-112.

Asare, A.T., Acheampong, E., Galyuon, I.K.A., Aboagye, L.M. and Takrama, J.F. 2011. Effects of sucrose on development of cultures of some accessions of Dioscorea spp. J. Ghana Sci.

Ass., 13 (2): 12-19.

Asha, K.I. and Nair, G.M. 2007. In vitro bulbil induction in Dioscorea species. J. Root Crops, 33 (2): 81-87.

Balakrishnan, V., Narayanan. M.K.R. and Anil Kumar, N. 2003. Ethnotaxonomy of Dioscorea among the Kattunaikka people

of Wayanad District, Kerala, India. Plant Genetic Resour. Newsl.,

: 24-32.

Chen, F.Q., Fu, Y., Wang, D.L., Gao, X. and Wang, L. 2007. The effect of plant growth regulators and sucrose on the

micropropagation and microtuberization of Dioscorea nipponica Makino. J. Plant Growth Regul., 26: 38-45.

Chu, E.P. and Ribeiro, R.C.L.F. 2002. Growth and carbohydrate changes in shoot cultures of Dioscorea species as influenced by photoperiod, exogenous sucrose and cytokinin concentrations.

Plant Cell Tissue Organ Cult., 70(3): 241-249.

Ezeibekwa, I.O., Ezenwaka, C.L., Mbagwu, F.N. and Unamba, C.I.N.

Effects of combination of different levels of auxin (NAA) and cytokinin (BAP) on in vitro propagation of Dioscorea rotundata L. (White Yam). New York Sci. J., 2(5): 1-8.

Forsyth, C. and Staden, V.J. 1982. An improved method of in vitro

propagation of Dioscorea bulbifera. Plant Cell Tissue Organ Cult., 1: 275-281.

George, E.F., Hall, M.A. and Klerk, G.J.D. 2008. Plant growth regulators II: Cytokinins, their analogues and antagonists. In: Plant Propagation by Tissue Culture. 3rd Edn., Volume 1. The Background. George, E.F., Hall, M.A. and Klerk, G.J.D. (Eds.) Springer, The Netherlands. pp. 205-226.

Islam, M.T., Keller, E.R.J. and Dembele, D.P. 2008. Effects of growth

regulators on in vitro propagation and tuberization of four Dioscorea species. Plant Tissue Cult. Biotech., 18(1): 25-35.

Jasik, J. and Mantell, S.H. 2000. Effects of jasmonic acid and its methyl ester on in vitro microtuberisation of three food yam (Dioscorea) species. Plant Cell Rep., 19: 863-867.

Klu, G.Y.P. 2002. Conservation of Dioscorea rotundata: effect of basal medium type and naphthalene acetic acid on growth and microtuberization. West Afr. J. Applied Ecology, 3: 99-103.

Lakshmisita, G., Bammi, R.K. and Randhawa, G.S. 1976. Clonal

propagation of Dioscorea floribunda by tissue culture. J. Hort.

Sci., 51: 551-554.

Lauzer, D., Laublin, G., Vincent, G. and Cappadocia, M. 1992. In vitro propagation and cytology of wild yams, Dioscorea

abyssinica Hoch. and D. mangenotiana Miege. Plant Cell Tissue Organ Cult., 28(2): 215-223.

Maheswari, R.U., Prabha, A.L., Nandagopalan, V. and Anburaja, V. 2012. In vitro rhizome production from nodal explants and

callus formation of the medicinal plant Dioscorea oppositifolia L. J. Pharmacy Biol. Sci., 1(6): 17-21.

Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant., 15: 473-497.

Narayanan, M.K.R., Anilkumar, N., Balakrishnan, V., Sivadasan, M.,

Alfarhan, H.A. and Alatar, A.A. 2011. Wild edible plants used by the Kattunaikka, Paniya and Kuruma tribes of Wayanad district, Kerala, India. J. Med. Plant Res., 5(15): 3520-3529.

Ng, S.Y.C.1988. In vitro tuberization in white yam (Dioscorea rotundata Poir.). Plant Cell Tissue Organ Cult., 14: 121-128.

Ovono. P.O., Kevers, C. and Dommes, J. 2007. Axillary proliferation

and tuberisation of Dioscorea cayenensis-D.rotundata complex. Plant Cell Tissue Organ Cult., 91: 107-114.

Poornima, G.N. and Ravishankar Rai, V. 2007. In vitro propagation

of wild yams, D. oppositifolia (Linn) and D. pentaphylla (Linn). African J. Biotechnol., 6(20): 2348-2352.

Prain, D. and Burkill, I.H. 1938. An account of the genus Dioscorea in the east. The species which twine to the right with addenda to part I and a summary, Royal Botanic Garden, Calcutta, part II.

SAS. 2010. SAS Institute, Inc., Cary, NC, USA.

Sengupta, J., Mitra, G.C. and Sharma, A.K. 1984. Organogenesis and tuberization in culture of Dioscorea floribunda. Plant Cell Tissue Organ Cult., 3(4): 325-331.

Thankappan, S.S. and Patell, V.M. 2011. In vitro propagation studies

and genetic fidelity assessment of endangered medicinal wild yam- Dioscorea prazeri. Plant Omics J., 4(4): 177-189.

Yan, H., Yang, L. and Li, Y. 2011. Axillary shoot proliferation and tuberisation of Dioscorea fordii Prain et Burk. Plant Cell Tissue Organ Cult., 104: 193-198.

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Published

2015-01-16

How to Cite

Thankappan, S., & K, A. (2015). In vitro propagation and microtuber induction in Dioscorea belophylla (Prain) Voigt ex Haine. JOURNAL OF ROOT CROPS, 40(1), 31–38. Retrieved from https://journal.isrc.in/index.php/jrc/article/view/230

Issue

Vol. 40 No. 1 (2014): Journal of Root Crops

Section

Research Articles

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