Evaluation of Culture Filtrate of an Entomopathogenic Bacterium for Nematicidal Properties Against Root-Knot Nematode, Meloidogyne incognita.
Keywords:
Meloidogyne incognita, nematicidal activity, root knot nematodes, SteinernemaAbstract
Bacterial culture, its culture filtrate and organic extract were tested invitro against infective juveniles (IJ) of Meloidogyne incognita. Nematicidal activity was observed in 24-96h exposure time with bacterial culture, cell free culture filtrate and organic crude extract at various concentrations. Mortality rate increased with concentration and exposure time. Bacterial culture filtrate using TSB, LB and NB media recorded 50%, 36.6% and 13.3% mortality respectively in 96h exposure time at a concentration of 150 µl/ml. Bacterial culture filtrate of TSB+Fructose exhibited nematicidal activity of 16.6% and 80% at a concentration of 150 µl/ml in 24 and 96h exposure time respectively which was higher than other media used. Organic crude extract obtained using TSB+Fructose media recorded a mortality of 70% and 100% at 2.5 µg/ml and 5 µg/ml concentration respectively in 24h exposure period.References
Abbott, W. S. 1925. A method of computing the effectiveness of an insecticide. J Econ Entomol., 18: 265-267. DOI: http://dx.doi.org/10.1093/jee/18.2.265a
Afzal, S., Tariq, S., Sultana, V., Ara, J. and Haque, S. E. 2013. Managing the root diseases of okra with endo-root plant growth promoting Pseudomonas and Trichoderma viride associated with healthy okra roots. Pak. J. Bot., 45(4): 1455-1460.1.
Castagnone.Sereno, P. 2002. Genetic variability in parthenogenetic root.knot nematodes, Meloidogyne spp. and their ability to overcome plant resistance genes. Nematology., 4: 605-608. DOI: 10.1163/15685410260438872
Cayrol, J. C., Djian, C. and Pijarowiski, L. 1989. Study on the nematicidal properties of the culture ï¬ltrate of the nematophagous fungus Paecilomyces lilacinus. Review Journal of Nematology., 12: 331.336.
Chen, G., Zhang, Y., Li, J., Dunphy, G. B., Punja, Z. K. and Webster, J. M. 1996. Chitinase activity of Xenorhabdus and Photorhabdus species, bacterial associates of entomopathogenic nematodes. Journal of Invertebrate Pathology., 68(2): 101-108. DOI:10.1006/JIPA.1996.0066
Hadad, M. E., Mustafa, M. I., Selim, S. M., Ahmed, E. A., Mahgoob, Tayeb, T. S. and Aziz, N. H. A. 2010. In vitro evaluation of some bacterial isolates as biofertilizers and biocontrol agents against the second stage juveniles of Meloidogyne incognita. World J Microbiol Biotechnol., 26: 2249.2256. DOI: 10.1007/s11274-010-0413-8
Huangin, H., Xu, C. K., Ma, L., Zhang,K. Q., Duan, C. Q. and Mo, M. H. 2010. Characterisation of volatiles produced from Bacillus megaterium YFM3.25 and their nematicidal activity against Meloidogyne incognita. Eur J Plant Pathol., 126: 417.422. DOI:10.1007/s10658-009-9550-z
Moens, M., Perry, R. N. and Starr, J. L. 2009. Meloidogyne species - a diverse group of novel and important plant parasites. In: Root-knot Nematodes. Perry, R.N., Moens, M. and Starr, J. L. (Eds.). CABI International, Cambrige, MA, USA: 1-17.
Nishanth Kumar, S., Siji, J. V., Ramya, R., Bala Nambisan and Mohandas, C. 2012. Improvement of antimicrobial activity of compounds Produced by Bacillus sp. associated with a Rhabditid sp. (entomopathogenic nematode) by changing carbon and
nitrogen sources in fermentation media. Journal of Microbiology, Biotechnology and Food Sciences., 1(6):1424-1438.
Racke, J. and Sikora, R. A. 1992. Isolation, formulation and antagonistic activity of rhizobacteria toward the potato cyst nematode Globodera pallida. Soil-Biol.- Biochem., 24(6): 521-526.
Roberts, P. A. 1992. Current status of the availability, development and use of host plant resistance to nematodes. Journal of Nematology., 24: 213.227.
Sadrati, N., Daoud, H., Zerroug, A., Dahamna, S. And Bouharati, S. 2013. Screening of antimicrobial and antioxidant Secondary metabolites from endophytic fungi isolated from wheat (Triticum durum). Journal of plant protection research vol. 53(2): 128.136. DOI: 10.2478/jppr-2013-0019
Sikora, R. A. and Fernandez, E. 2005. Nematode parasites of vegetables. In: Plant Parasitic Nematodes in Subtropical and Tropical Agriculture (Second edition). Edited by: Luc, M., Sikora, R.A and Bridge, A.: 319-384.
Zeinat, Kamel, M., Atef, N. M., El-Sayed, S. A. and Wahab, A. E. G. S. 2010. Optimization of microbial biomass production as biocontrol agent against root knot nematode on faba plants, Journal of American Science., 6(6): 245-255.
Downloads
Published
How to Cite
Issue
Section
License
Dear Author(s)
We need your written permission before we can publish your accepted article in the Journal of Root Crops. Please read this form carefully, complete it and palace your signature below. A scanned copy of this form may be mailed to us within two days of receipt of this letter. Our email ID is: isrc_ctcri@yahoo.com or editor_jrc@yahoo.com or sreekumarctcri@gmail.com
Details:
In consideration of the publication in the Journal of Root Crops of our contribution entitled
(Name of the paper): .................................................................................................................
.................................................................................................................................................... by us (all Authors’ names) ..........................................................................................................
....................................................................................................................................................
A. To be filled in if copyright belongs to you
I/we hereby assign to Indian Society for Root Crops, Thiruvananthapuram, India, the full copyright in all forms and media in the said contribution, including open/ restricted access to online version. They own the rights to freely grant or distribute the paper to any third party or public
Please tick this box if you are the sole author of the paper
Please tick this box if you are authorised to execute this copyright transfer on behalf of all the authors of the above article
Name (block letters): . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .......
Institution/Company:..................................................................................................................
....................................................................................................................................................
Signature:.....................................................................; Date: ....................................................
B. To be filled in if copyright does not belong to you
1. Name and address of copyright holder:................................................................................
........................................................................................................................................................................................................................................................................................................
2. The copyright holder hereby grants Indian Society for Root Crops, Thiruvananthapuram, India the full copyright in all forms and media in the said contribution, including open/ restricted access to online version. They own the rights to freely grant or distribute the paper to any third party or public.
(Signature of copyright holder or authorised agent)
