Hot Water Treatment: An Efficient Method for Elimination of Yam Mild Mosaic Virus in Dioscorea alata

Authors

  • Dhanya Jayaseelan Central Tuber Crops Research Institute, Sreekariyam, Thiruvananthapuram- 695 017, Kerala, India
  • M Rajitha Central Tuber Crops Research Institute, Sreekariyam, Thiruvananthapuram- 695 017, Kerala, India
  • M.K. Dhanya Central Tuber Crops Research Institute, Sreekariyam, Thiruvananthapuram- 695 017, Kerala, India
  • Vinayaka Hegde Central Plantation Crops Research Institute, Kasaragodu, Kerala, India
  • M.L. Jeeva Central Tuber Crops Research Institute, Sreekariyam, Thiruvananthapuram- 695 017, Kerala, India

Abstract

Greater yam (  Dioscorea alata) is an important food crop in the Southern and North-Eastern states of India. Yams are prone to diseases. Among these, the  Yam mosaic virus (YMV) and Yam mild mosaic virus  (YMMV) belonging to the potyvirus group are widely prevalent. Hot water treatment is one of the methods of heat treatment in which the nodes are immersed in hot water for different time durations. With an aim to eliminate virus, hot water treatment of nodes has been tried to develop virus free planting materials through tissue culture. The vine cuttings (2-3 cm) of  Dioscorea alata var. Sree Karthika with single node were treated with hot water at 32oC and 36oC for different time durations of 10, 20 and 30 minutes. The YMMV elimination was confirmed at regular intervals by RT-PCR with YMMV specific primers. Treatment of nodes at 36oC for 30 minutes was found to be the most efficient in virus elimination (90%), followed by 32oC for 30 minutes.

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Published

2013-07-23

How to Cite

Jayaseelan, D., Rajitha, M., Dhanya, M., Hegde, V., & Jeeva, M. (2013). Hot Water Treatment: An Efficient Method for Elimination of Yam Mild Mosaic Virus in Dioscorea alata. JOURNAL OF ROOT CROPS, 37(1), 60–64. Retrieved from https://journal.isrc.in/index.php/jrc/article/view/11

Issue

Vol. 37 No. 1 (2011): Jounal of Root Crops 37(1)

Section

Research Articles

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