Optimization of Media for Antimicrobial Activity of Enterobacter sp. Associated with Entomopathogenic Nematode Rhabditid sp.

Authors

  • B G Sangeetha CTCRI
  • C A Jayaprakas
  • J R Jithine
  • C Mohandas

Keywords:

Antimicrobial activity, entomopathogenic, Rhabditis ( Oscheius ), tryptic soya broth

Abstract

An entomopathogenic bacterium isolated from the nematode,Rhabditis(Oscheius) sp. was found toproduce secondary metabolites with antimicrobial activity. Media for the production of the bioactivemetabolites were standardized with tryptic soya broth (TSB) supplemented with six carbon sources viz.,glycerol, maltose, fructose, glucose, sucrose and lactose. Antimicrobial activity was found highest forculture filtrate solvent extract (CFSE) obtained from TSB plus glycerol combination. Among the differentcarbon sources tested, glycerol proved to be the best for antimicrobial activity and that was followed bymaltose, fructose and glucose. Media with lactose and sucrose combination had the least antimicrobialactivity. A high degree of variation in the level of antimicrobial activity against the test organism viz.,Fusarium oxysporumMTCC 284,Rhizoctonia solaniMTCC 4634,Penicillium expansumMTCC2006,Aspergillus flavusMTCC 183,Candida albicansMTCC 277 was observed. The present study revealedthat the source of carbon has a pivotal role in the production of antimicrobial activity.

Downloads

Published

2017-05-06

How to Cite

Sangeetha, B. G., Jayaprakas, C. A., Jithine, J. R., & Mohandas, C. (2017). Optimization of Media for Antimicrobial Activity of Enterobacter sp. Associated with Entomopathogenic Nematode Rhabditid sp. JOURNAL OF ROOT CROPS, 42(2), 121–127. Retrieved from https://journal.isrc.in/index.php/jrc/article/view/427

Issue

Vol. 42 No. 2 (2016): Journal of Root Crops, Vol 42(2)

Section

Research Articles

License

Dear Author(s)

We need your written permission before we can publish your accepted article in the Journal of Root Crops.   Please read this form carefully, complete it and palace your signature below. A scanned copy of this form may be mailed to us within two days of receipt of this letter. Our email ID is: isrc_ctcri@yahoo.com  or    editor_jrc@yahoo.com  or sreekumarctcri@gmail.com 

 

Details:

In consideration of the publication in the Journal of Root Crops of our contribution entitled

(Name of the paper): .................................................................................................................

.................................................................................................................................................... by us (all Authors’ names) ..........................................................................................................

....................................................................................................................................................

 
   

 

 

A.  To be filled in if copyright belongs to you

                      I/we hereby assign to Indian Society for Root Crops, Thiruvananthapuram, India, the full copyright in all forms and media in the said contribution, including open/ restricted access to online version. They own the rights to freely grant or distribute the paper to any third party or public

    Please tick this box if you are the sole author of the paper

    Please tick this box if you are authorised to execute this copyright transfer on behalf of all the authors of the above article

 

Name (block letters): . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .......

Institution/Company:..................................................................................................................

....................................................................................................................................................

Signature:.....................................................................; Date: ....................................................

 
   

 

 

B. To be filled in if copyright does not belong to you

1. Name and address of copyright holder:................................................................................

........................................................................................................................................................................................................................................................................................................

 

2. The copyright holder hereby grants Indian Society for Root Crops, Thiruvananthapuram, India the full copyright in all forms and media in the said contribution, including open/ restricted access to online version. They own the rights to freely grant or distribute the paper to any third party or public.

 

 (Signature of copyright holder or authorised agent)