Genetic Diversity of Phytophthora colocasiae Causing Taro Leaf Blight: Analysis Using Start Codon Targeted (SCoT) Polymorphism

Authors

  • Vishnu Sukumari Nath Division of crop protection Central Tuber Crops Research Institute
  • Vinayaka Mahabaleswar Hegde Division of crop protection Central Plantation Crops Research Institute
  • Muthulekshmi Lajapathy Jeeva Division of crop protection Central Tuber Crops Research Institute
  • Raj Shekar Misra Regional centre, Central Tuber Crops Research Institute
  • Syamala Swayamvaran Veena Division of crop protection Central Tuber Crops Research Institute
  • Mithun Raj Division of crop protection Central Tuber Crops Research Institute
  • Sree Sankar Dharveekaran Nair Division of crop protection Central Tuber Crops Research Institute

Keywords:

Taro, Phytophthora colocasiae, Leaf blight, SCoT marker, Genetic diversity, disease management

Abstract

The Oomycetous fungus Phytophthora colocasiae that causes taro leaf blight is one of the most devastating diseases of taro and is widely distributed in India. Cultural and molecular techniques were employed for assessing the genetic variability among 30 isolates of P. colocasiae obtained from different geographical origins of India. Cultural characters like pathogenicity assay, mating type and metalaxyl sensitivity showed variation among isolates. Eight Start Codon Targeted Polymorphism (SCoT) markers produced 121 reproducible fragments with 100% polymorphism. The average value of the number of observed alleles, the number of effective alleles, mean Nei’s genetic diversity, and Shannon’s information index were 2.00 ± 0.00, 1.58 ± 0.30, 0.34 ± 0.13, and 0.51 ± 0.16, respectively. Analysis of molecular variance (AMOVA) showed that 89 % of the diversity was present within population of P. colocasiae. Dendrograms based on the molecular data using the unweighted pair group method with arithmetic mean (UPGMA) classified the P. colocasiae isolates into two major clusters. Cophenetic correlation coefficient between dendrogram and original similarity matrix were significant for SCoT marker (r = 0.904). The results of this study displayed a high level of genetic variation among the isolates irrespective of the geographical origin. The possible mechanisms and implications of this genetic variation are discussed.

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Published

2015-01-01

How to Cite

Nath, V. S., Hegde, V. M., Jeeva, M. L., Misra, R. S., Veena, S. S., Raj, M., & Nair, S. S. D. (2015). Genetic Diversity of Phytophthora colocasiae Causing Taro Leaf Blight: Analysis Using Start Codon Targeted (SCoT) Polymorphism. JOURNAL OF ROOT CROPS, 39(2), 168–177. Retrieved from https://journal.isrc.in/index.php/jrc/article/view/76

Issue

Vol. 39 No. 2 (2013): Journal of Root Crops

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Research Articles

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