Elimination of Cassava Mosaic Disease through Meristem Culture and Field Evaluation for Yield Loss Assessment in Cassava Genotypes

Authors

  • D C Deepthi CTCRI
  • T Makeshkumar

Keywords:

Cassava, meristem culture, SLCMV, PCR, yield

Abstract

The presence ofSri Lankan cassava mosaic virus(SLCMV) andIndian cassava mosaic virus(ICMV) andother cassava mosaic viruses which cause cassava mosaic disease is a major threat to cassava productionworldwide. Therefore it is highly advantageous to use virus free cuttings for multiplication and cultivation.Here we report meristem culture for cleaning of five different cassava cultivars infected with SLCMV.Polymerase chain reaction (PCR) was employed to index meristem derived plants using specific primers.The meristem derived plantlets were free of symptoms and further confirmed the absence of virusthrough PCR using virus specific primers. Meristem derived plants were included in field study to determinethe effect of SLCMV infection in yield. Meristem derived virus free planting materials increases yield butsuch advantage soon lost due to rapid re-infection with virus on field grown plants through efficientwhitefly transmission.

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Published

2016-12-27

How to Cite

Deepthi, D. C., & Makeshkumar, T. (2016). Elimination of Cassava Mosaic Disease through Meristem Culture and Field Evaluation for Yield Loss Assessment in Cassava Genotypes. JOURNAL OF ROOT CROPS, 42(1), 45–52. Retrieved from https://journal.isrc.in/index.php/jrc/article/view/397

Issue

Vol. 42 No. 1 (2016): Journal of Root Crops Vol42(1)

Section

Research Articles

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