Evaluation of Intra and Inter-specific Variability among Species of Curcuma using Microsatellite Markers

Authors

  • Bimal Thomas Department of Plant Biotechnology, College of Agriculture, Vellayani-695 522
  • KI Asha Division of Crop Improvement, ICAR-Central Tuber Crops Research Institute, Sreekariyam, Thiruvananthapuram-695 017
  • N Krishna Radhika Division of Crop Improvement, ICAR-Central Tuber Crops Research Institute, Sreekariyam, Thiruvananthapuram-695 017

Abstract

Microsatellite markers such as ISSRs and SSRs were used to study the genetic variability among 15 selected accessions in 8 species of Curcuma germplasm comprising of two accessions in each of the seven Curcuma species and one of C. longa. Of the primers tested, 10 (out of 10) ISSR and 7 (out of 10) SSR primers were selected for their reproducibility and high polymorphism. A total of 166 polymorphic ISSR bands were detected out of 176 bands and a total of 123 polymorphic SSR bands were detected out of 135 bands. Results demonstrated an extensive genetic variability within the selected accessions as well as the value of ISSR and SSR markers in characterization of Curcuma species. Marker scoring data was used to construct UPGMA dendrogram and Pearson Correlation Coefficient. For both markers a high similarity in dendrogram clustering was obtained. All dendrograms, including that obtained by the combined use of both the ISSR and SSR marker data, depicted the genetic relationship/variability among the accessions, depending upon their species similarity/dissimilarity.

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Published

2018-03-31

How to Cite

Thomas, B., Asha, K., & Krishna Radhika, N. (2018). Evaluation of Intra and Inter-specific Variability among Species of Curcuma using Microsatellite Markers. JOURNAL OF ROOT CROPS, 44(1), 12–18. Retrieved from https://journal.isrc.in/index.php/jrc/article/view/509

Issue

Vol. 44 No. 1 (2018): Journal of Root Crops Vol 44 (1)

Section

Research Articles

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