Influence of Age of Explants and Genotype on Somatic Embryogenesis in African and Indian Cassava Cultivars

Authors

  • Beena M Ravindran SRF, CTCRI Present Address Post Doctoral Fellow, DSMZ, Braunschweig-38104
  • Stephan Winter Head, Plant Virology Department, Leibniz Institute-DSMZ, Braunschweig-38104
  • Makeshkumar Thangaraj Principal Scientist, Central Tuber Crops Research Institute, Sreekariym, Trivandrum - 695017

Keywords:

Cassava, African & Indian cultivar, embryogenic callus, somatic embryos

Abstract

Potential of eight cassava cultivars viz., TME3, TME4, TMS 30572, TMS 1089A, 96/0160, Albert,Kibaha and H165 for initiating somatic embryos was studied. Two different explants, young leaf lobe pre-cultured on Murashige and Skoog (MS) medium and axillary bud pre-cultured on MS medium containing BAP were tried. The age of explants and type of cultivar highly influenced the frequency and quantity of somatic embryos. Axillary buds of cv. Albert and young leaf lobes of cv. H165 responded well, while the same results were observed for conversion and development. Hardening and establishment in green house was close to 100% for all the cultivars under study. This paper discusses the effect of juvenility of the explants used, duration of pre-treatments and culture on embryogenesis medium, cultivar and its preference of explant, health of the mother plants, on successful regeneration of cassava via somatic embryogenesis, which could be very resourceful for friable embryogenic calli production and successful genetic transformation.

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Published

2015-01-19

How to Cite

Ravindran, B. M., Winter, S., & Thangaraj, M. (2015). Influence of Age of Explants and Genotype on Somatic Embryogenesis in African and Indian Cassava Cultivars. JOURNAL OF ROOT CROPS, 40(2), 21–27. Retrieved from https://journal.isrc.in/index.php/jrc/article/view/161

Issue

Vol. 40 No. 2 (2014): Journal of Root Crops

Section

Research Articles

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